Figure 4
From: ADDME – Avoiding Drug Development Mistakes Early: central nervous system drug discovery perspective
Formation of reactive metabolites of acetaminophen. Acetaminophen (ACET) was incubated with microsomes and glutathione (GSH) in the presence and absence of NADPH. An adduct of glutathione with acetaminophen was formed in the presence of NADPH. When NADPH was absent (no reaction control), no adduct was formed.