Fig. 1

Hypoxic stress affected glucose metabolism and the expression of miR-18a in HT22 cells. (A) Analysis of differentially expressed miRNAs in hypoxia and hypoxia related studies. (B) The effect of hypoxic stress on lactic acid production was detected. n = 4, **: p < 0.01. (C) qPCR was used to detect the expression of Ldha, Slc2a1, Gapdh and Pdk1 genes in HT22 cells after hypoxia stress. The Actb gene was used as the internal reference gene (the protein corresponding to the Actb gene is β-actin). n = 3, *: p < 0.05, **: p < 0.01. (D-E) Western blot was used to detect the expression of Gapdh and Glut1 after 24 h of hypoxic stress. β-actin was used as an internal reference protein. (F) The expression of miR-18a in HT22 cells after hypoxic stress was detected by qPCR. U6 gene was used as the internal reference gene. n = 3, *: p < 0.05