Fig. 2

MiR-18a regulated the expression of genes related to glycolysis. (A) The expression of the Gapdh gene and Pdk1 gene was detected by qPCR after transfection of miR-18a mimics and negative control (NC) for 24 h and hypoxic stress for 12 h. The Actb gene was used as the internal reference gene. n = 3, **: p < 0.01. (B) Western blot was used to detect the expression of Gapdh after transfection of miR-18a mimics and NC for 24 h and hypoxic stress for 12 h. β-actin was used as an internal reference protein. (C) The expression of the Gapdh gene and Pdk1 gene was detected by qPCR after transfection of miR-18a mimics and NC for 36 h. The Actb gene was used as the internal reference gene. n = 3, *: p < 0.05. (D) Western blot was used to detect the expression of Gapdh after transfection of miR-18a mimics and negative control for 36 h. β-actin was used as an internal reference protein