Fig. 4

MiR-18a inhibited the expression of the Hif1a gene. (A) Phylogenetic tree constructed from the 3’UTRs of the Hif1a gene in multiple species. Analysis of miR-18 binding sites and flanking sequences in the 3’ UTRs of the Hif1a gene in multiple species. (B) The expression of the Hif1a gene was detected by qPCR after transfection of miR-18a mimics and NC for 24 h and hypoxic stress for 12 h. The Actb gene was used as the internal reference gene. n = 3, **: p < 0.01. (C) Western blot was used to detect the expression of Hif-1α after transfection of miR-18a mimics and NC for 24 h and hypoxic stress for 12 h. β-actin was used as an internal reference protein