Fig. 5

MiR-18a regulated the 3’UTR of the Hif1a gene. (A) Schematic of the psi-3’UTR-Hif1a vector and the psi-3’UTR-mut-Hif1a vector construction. (B) Schematic of the complementary sites between psi-3’UTR-Hif1a vector and psi-3’UTR-mut-Hif1a vector with the miR-18a seed sequence. (C) 24 h after co-transfection of miR-18a mimics (or NC) and the corresponding vector, the relative activity luciferase was detected. n = 4, **: p < 0.01. (D) 24 h after co-transfection of miR-18a inhibitor (or inhibitor negative control, lNC) and the corresponding vector, the relative activity luciferase was detected. n = 4, *: p < 0.05