Fig. 6

Hif-1α affected the expression of metabolism related genes. (A) Western blot was used to detect the expression of Hif-1α in HT22 cells after 12 h of hypoxic stress. (B) qPCR was used to detect the expression of the Hif1a gene after transfection of siRNA for 24 h and hypoxic stress for 12 h. The Actb gene was used as the internal reference gene. n = 3, **: p < 0.01. (C) Western blot was used to detect the expression of Hif-1α and Gapdh after transfection of siRNA for 24 h and hypoxic stress for 12 h. β-actin was used as an internal reference protein. (D) The Gapdh gene and Pdk1 gene was detected by qPCR after transfection of siRNA for 24 h and hypoxic stress for 12 h. The Actb gene was used as the internal reference gene. n = 3, **: p < 0.01