Fig. 4

Reconstituition kinetics of CSF parameters under antifungal therapy. Slow normalization of standard CSF parameters and variable courses of NK cell populations after induction of antifungal therapy. Parameters over the course of time of the CM/HIV+ group are represented as lines in green. Lines of the CM/HIV- group are represented in red color. Flow cytometry of CSF was performed at the first presentation of each patient and during follow-up in different intervals. Antifungal therapy was started within days after the first diagnosis of CM. Samples were taken in different time intervals, trends of each parameter are represented over a period of 150 days. First sample (day 0) of two patients were taken during follow up, both were already on antifungal therapy (Patient 10: 10 months after first diagnosis; Patient 24: 30 months after first diagnosis). Correlation analysis was performed with Pearson’s correlation coefficient and p values were adjusted with the Benjamini–Hochberg method. A Reconstitution kinetics of cells, protein and immunoglobulins in CSF. B Reconstitution kinetics of plasma cells, NK cells and NK subtypes. C Reconstitution kinetics of T cell, monocytes and monocyte subpopulations. Abbreviations - Bc: B lymphocytes, BCBD: blood-CSF-barrier disruption, brightNK: CD56^bright natural killer cells, CM: cryptococcal meningitis, cMono: classical monocytes, CSF: cerebrospinal fluid, Ctrl: healthy control group, dimNK: CD56^dim natural killer cells, Granulo: proportion of granulocytes, HIV: human immunodeficiency virus positive, immunocompromised control group, HLA-DR Tc: activated T cells, IgA: immunoglobulin A,  IgG: immunoglobulin G,  IgM: immunoglobulin M,  iMono: intermediate monocytes, Lympho: proportion of lymphocytes, mono: monocytes, ncMono: non-classical monocytes, NK: natural killer cells, NKT: natural killer T cells, OCB: oligoclonal bands, Plasma: plasma cells, ROC: receiver operating characteristic analysis, Tc: T lymphocytes