Fig. 8

This is the representative photomicrograph of the hippocampus which shows the effect of QC treatment on CA1 region (H&E staining, magnification 400x). A Intact; B Sham; C MCAO; D Vehicle; E QC 5 mg/kg; F QC 10 mg/kg; G QC 20mg/kg ; H CA1 region (magnification 40x). Yellow arrow indicates normal neurons; Black arrow indicates apoptotic neurons. In the control groups [Intact (A), sham (B)]: pyramidal cells with round nuclei, prominent nucleoli and clear cytoplasm are visible. In MCAO and Vehicle groups [(C), (D)]: apoptotic cells with highly dense, granular and wrinkled nucleus, indistinct nuclei and cytoplasm are observed with increased staining. In treatment groups: E In 5 mg/kg dose, normal cells with clear nuclei are observed along with damaged cells with granulated and pigmented nuclei. F In 10 mg/kg dose, normal cells with clear nuclei are observed along with a few damaged cells with granulated and pigmented nuclei. G In 10 mg/kg dose, apoptotic cells with highly dense, granular and wrinkled nucleus, indistinct nuclei and cytoplasm are observed with increased staining. H Overview of the hippocampus and the CA1 area